Molded flow channel
A nanopore based sequencing system includes a plurality of nanopore sensors. Each nanopore sensor has a portion for receiving a fluid. The nanopore based sequencing system includes a fluid chamber configured to guide the fluid over the plurality of nanopore sensors and an inlet configured to deliver the fluid into the fluid chamber. At least a portion of the fluid chamber is made of a material that has been molded around at least a portion of an electrode.
1 . A nanopore based sequencing system, the system comprising:
a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a cell and a working electrode, wherein the cell is configured to support a membrane and a nanopore disposed in the membrane, wherein the nanopore is a protein nanopore;
a counter electrode;
a flow channel disposed over the nanopore sensor array;
an inlet configured to deliver the fluid into the flow channel, wherein the inlet is located proximate a first side of the nanopore sensor array; and
an outlet configured to deliver fluid out of the flow channel, wherein the outlet is also located proximate the first side of the nanopore sensor array.
2 . The nanopore based sequencing system of claim 1 , wherein the nanopore sensory array comprises one million nanopore sensors.
3 . The nanopore based sequencing system of claim 1 , wherein the protein nanopore is attached to a polymerase.
4 . The nanopore based sequencing system of claim 3 , wherein the polymerase is associated with a nucleic acid molecule to be sequenced.
5 . The nanopore based sequencing system of claim 4 , wherein the nucleic acid molecule is circular.
6 . The nanopore based sequencing system of claim 1 , wherein the flow channel is horseshoe shaped.
7 . A nanopore based sequencing system, the system comprising:
a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a cell and a working electrode;
a counter electrode;
a flow channel disposed over the nanopore sensor array, wherein the flow channel is serpentine shaped;
an inlet configured to deliver the fluid into the flow channel, wherein the inlet is located proximate a first side of the nanopore sensor array; and
an outlet configured to deliver fluid out of the flow channel, wherein the outlet is also located proximate the first side of the nanopore sensor array.
8 . A nanopore based sequencing system, the system comprising:
a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a cell and a working electrode;
a counter electrode;
a flow channel disposed over the nanopore sensor array, wherein the flow channel comprises at least one fluid guide;
an inlet configured to deliver the fluid into the flow channel, wherein the inlet is located proximate a first side of the nanopore sensor array; and
an outlet configured to deliver fluid out of the flow channel, wherein the outlet is also located proximate the first side of the nanopore sensor array.
9 . A nanopore based sequencing system, the system comprising:
a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a cell and a working electrode, wherein the cell is configured to support a membrane and a nanopore disposed in the membrane, wherein the nanopore is attached to a polymerase,
wherein the polymerase is associated with a nucleic acid molecule to be sequenced, wherein the nucleic acid molecule is circular;
a counter electrode;
a flow channel disposed over the nanopore sensor array;
an inlet configured to deliver the fluid into the flow channel, wherein the inlet is located proximate a first side of the nanopore sensor array; and
an outlet configured to deliver fluid out of the flow channel, wherein the outlet is also located proximate the first side of the nanopore sensor array.
10 . The nanopore based sequencing system of claim 9 , wherein the nanopore sensory array comprises one million nanopore sensors.
11 . The nanopore based sequencing system of claim 9 , wherein the flow channel is horseshoe shaped.
12 . The nanopore based sequencing system of claim 9 , wherein the flow channel is serpentine shaped.
13 . The nanopore based sequencing system of claim 9 , wherein the flow channel comprises at least one fluid guide.
14 . A nanopore based sequencing system, the system comprising:
a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a cell and a working electrode, wherein the cell is configured to support a membrane and a nanopore disposed in the membrane, wherein the nanopore is attached to a polymerase, wherein the polymerase is associated with a nucleic acid molecule to be sequenced, wherein the nucleic acid molecule is circular;
a counter electrode;
a flow channel disposed over the nanopore sensor array, wherein the flow channel is horseshoe shaped;
an inlet configured to deliver the fluid into the flow channel, wherein the inlet is located proximate a first side of the nanopore sensor array; and
an outlet configured to deliver fluid out of the flow channel, wherein the outlet is also located proximate the first side of the nanopore sensor array.
15 . The nanopore based sequencing system of claim 14 , wherein the nanopore sensory array comprises one million nanopore sensors.
16 . A nanopore based sequencing system, the system comprising:
a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a cell and a working electrode, wherein the cell is configured to support a membrane and a nanopore disposed in the membrane, wherein the nanopore is attached
to a polymerase, wherein the polymerase is associated with a nucleic acid molecule to be sequenced, wherein the nucleic acid molecule is circular;
a counter electrode;
a flow channel disposed over the nanopore sensor array, wherein the flow channel is serpentine shaped;
an inlet configured to deliver the fluid into the flow channel, wherein the inlet is located proximate a first side of the nanopore sensor array; and
an outlet configured to deliver fluid out of the flow channel, wherein the outlet is also located proximate the first side of the nanopore sensor array.
17 . The nanopore based sequencing system of claim 16 , wherein the nanopore sensory array comprises one million nanopore sensors.