Method of electrowetting
A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the conformal layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the matrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.
1 . A method of forming an electrokinetic device for moving an aqueous droplet, comprising:
providing a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor;
coating the matrix electrodes are overcoated with a functional coating comprising:
one or more dielectric layer(s) comprising silicon nitride, hafnium oxide or aluminum oxide in contact with the matrix electrodes,
a conformal layer comprising parylene in contact with the dielectric layer, and
a hydrophobic layer in contact with the conformal layer;
providing a second substrate comprising a top electrode; and
disposing a spacer between the first substrate and the second substrate to define an electrokinetic workspace.
2 . The method of claim 1 , further comprising coating the matrix electrodes with a plurality of layer(s) comprising silicon nitride, hafnium oxide or aluminium oxide in contact with the matrix electrodes.
3 . The method of claim 1 , wherein the dielectric layer is between 10 nm and 100 μm thick.
4 . The method of claim 1 , wherein the layered dielectric comprises:
a first layer including an aluminum oxide or a hafnium oxide, the first layer having a thickness between 9 nm and 80 nm;
a second layer including a tantalum oxide or a hafnium oxide, the second layer having a thickness between 40 nm and 250 nm; and
a third layer including a tantalum oxide or a hafnium oxide, the third layer having a thickness between 5 nm and 60 nm, wherein the second layer is disposed between the first and third layers.
5 . The method of claim 1 , wherein the conformal layer is between 10 nm and 100 μm thick.
6 . The method of claim 1 , wherein the hydrophobic layer comprises any of a fluoropolymer coating, a fluorinated silane coating, manganese oxide polystyrene nanocomposite, zinc oxide polystyrene nanocomposite, precipitated calcium carbonate, a carbon nanotube structure, a silica nanocoating, or a slippery liquid-infused porous coating.
7 . The method of claim 1 , wherein the functional coating includes a dielectric layer comprising silicon nitride, a conformal layer comprising parylene, and a hydrophobic layer comprising an amorphous fluoropolymer.
8 . The method of claim 1 , further comprising coupling a controller to the electrokinetic device to regulate a voltage provided to the individual matrix electrodes.
9 . The method of claim 8 , wherein the electrokinetic device further includes a plurality of scan lines and a plurality of gate lines, wherein each of the thin film transistors is coupled to a scan line and a gate line, and the plurality of gate lines are operatively connected to the controller.
10 . The method of claim 1 , further comprising coating the top electrode with a second hydrophobic layer disposed on the second electrode.
11 . The method of claim 10 , wherein the first and second substrates are disposed so that the hydrophobic layer and the second hydrophobic layer face each other, thereby defining the electrokinetic workspace between the hydrophobic layer and the second hydrophobic layer.
12 . A method for performing a droplet based assay on an electrokinetic device formed by the method of claim 1 , wherein the method comprises repeatedly providing a differential electrical potential between a first of the matrix electrodes and a second of the matrix electrodes with a voltage source, thereby moving an aqueous droplet between the first of the matrix electrodes and the second of the matrix electrodes.
13 . The method of claim 12 , further comprising repeating the steps of claim 12 in order to add nucleotides to an initiation oligonucleotide.
14 . The method of claim 12 , further comprising repeating the steps of claim 12 in order to amplify nucleic acids within one or more droplets.
15 . The method of claim 12 , further comprising repeating the steps of claim 1 in order to join two or more nucleic acid strands in one or more droplets.
16 . The method of claim 12 , wherein the aqueous droplet is moved between the first matrix electrode and the second matrix electrode more than 1000 times.
17 . The method of claim 12 , wherein the aqueous droplet has an ionic strength greater than 0.1 M.
18 . The method of claim 12 , wherein the aqueous droplet has a volume of 1 μL or smaller.
19 . The method of claim 12 , further comprising:
disposing a second aqueous droplet on a third matrix electrode; and
providing a differential electrical potential between the third matrix electrode and the second matrix electrode with the voltage source, thereby contacting the aqueous droplet with the second aqueous droplet.
20 . The method of claim 1 , further comprising coupling a voltage source to the matrix electrodes.