Granted Patent B2

US 12,654,389 · App. 17/105,218 · Granted Jun 16, 2026

Inventor: Melanie P. Matheu (San Francisco, CA)

Assignee: PRELLIS BIOLOGICS, INC.

B29C64/135A61L27/26A61L27/3604A61L27/3612A61L27/362A61L27/3625A61L27/3633A61L27/3683A61L27/3804A61L27/3886A61L27/50A61L27/507A61L27/60B29C64/277B29C64/393B33Y10/00B33Y50/02B33Y70/00B33Y70/10C12M21/08C12M33/00C12N5/0062C12N5/06C12N5/0697B29K2033/08B29K2105/0035B33Y80/00

Abstract

Claims (27)

1 . A system for printing a three-dimensional (3D) object, comprising:

a media chamber that contains a medium comprising one or more polymer precursors;

at least one pulsed energy source to output at least one pulsed energy beam to said media chamber; and

one or more computer processors operatively coupled to said at least one pulsed energy source, wherein said one or more computer processors are individually or collectively programmed to

(i) receive computer instructions for printing said 3D object from computer memory;

(ii) receive a computer model of the 3D object in the computer memory; and

(iii) in accordance with said computer instructions and the computer model of the 3D object, direct said at least one pulsed energy source to output said at least one pulsed energy beam into said medium, wherein the at least one pulsed energy beam simultaneously generates a plurality of points or lines patterned as a 3D holographic projection of multiple layers of said 3D object in a volume of said medium, thereby subjecting said polymer precursors to form a polymer matrix at the multiple layers at the same time to form said 3D object.

2 . The system of claim 1 , wherein said media chamber further comprises a plurality of cells.

3 . The system of claim 2 , wherein said plurality of cells comprises cells of at least two different types.

4 . The system of claim 2 , wherein said 3D object comprises at least one cell of said plurality of cells.

5 . The system of claim 1 , wherein said one or more computer processors are individually or collectively programmed to generate a point-cloud representation or lines-based representation of the computer model of said 3D object in computer memory, and use said point-cloud representation or lines-based representation to generate said computer instructions for printing said 3D object in computer memory.

6 . The system of claim 5 , wherein said one or more computer processors are individually or collectively programmed to convert said point-cloud representation or lines-based representation into an image.

7 . The system of claim 6 , wherein said one or more computer processors are individually or collectively programmed to project said image in a holographic manner.

8 . The system of claim 1 , wherein said at least one pulsed energy source is a plurality of pulsed energy sources.

9 . The system of claim 8 , wherein said at least one pulsed energy beam is a plurality of pulsed energy beams, and wherein said plurality of pulsed energy sources output said plurality of pulsed energy beams.

10 . The system of claim 1 , wherein said at least one pulsed energy source is a laser.

11 . The system of claim 1 , wherein said at least one pulsed energy source comprises a coherent light source.

12 . The system of claim 11 , wherein said coherent light source comprises a wavelength from about 300 nm to about 5 mm.

13 . The system of claim 1 , wherein said one or more computer processors are individually or collectively programmed to direct said at least one pulsed energy source to output at least one additional pulsed energy beam to form another 3D object.

14 . The system of claim 13 , wherein said at least one additional energy beam is along an x axis, an x and y plane, or x, y, and z planes.

15 . The system of claim 1 , further comprising at least one objective lens that direct said at least one pulsed energy beam to said medium in said media chamber.

16 . The system of claim 15 , wherein said at least one objective lens comprises a water dipping objective lens.

17 . The system of claim 1 , wherein said one or more computer processors are individually or collectively programmed to receive images of at least one edge of said 3D object.

18 . The system of claim 17 , wherein said one or more computer processors are individually or collectively programmed to direct linking of said 3D object with a tissue, which linking is in accordance with said computer instructions.

19 . The system of claim 1 , further comprising at least one port that facilitates addition or removal of said medium into said media chamber.

20 . The system of claim 1 , further comprising at least one port that facilitates addition or removal of at least one cell to said medium or into said 3D object subsequent to formation of said 3D object.

21 . The system of claim 1 , wherein said system does not include a digital micromirror device.

Assignments (1)

Continuity (4)

References Cited (233)