Microfluidic apparatus and methods of use thereof
Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.
1 . A microfluidic path device comprising:
(a) a first layer;
(b) a second layer;
(c) a third layer interposed between the first layer and the second layer, the third layer including an elastic membrane;
(d) a plurality of chambers formed between the first layer and the second layer, a portion of the elastic membrane dividing each chamber into a fluid-contacting side in the first layer and a pressure-receiving side in the second layer;
(e) a plurality of fluid channels providing paths for communication of fluid among each fluid-contacting side of the plurality of chambers; and
(f) a plurality of pressure ports providing paths for communication of pneumatic pressure to each pressure receiving side of the plurality of chambers,
the plurality of chambers including a vacuum cap, the vacuum cap being in fluid communication with the fluid-contacting side of one or more other chambers of the plurality of chambers, the vacuum cap being configured to provide removal of air from fluid communicated from the fluid-contacting side of the one or more other chambers in fluid communication with the vacuum cap, the vacuum cap including at least one projection configured to prevent the elastic membrane from sealing against at least one side of the vacuum cap.
2 . The microfluidic path device of claim 1 , further comprising a plurality of fluid ports configured to provide paths for communication of fluid through the plurality of fluid channels.
3 . The microfluidic path device of claim 2 , the plurality of fluid ports being positioned in the first layer.
4 . The microfluidic path device of claim 2 , the third layer including a plurality of fluid openings, each fluid opening being positioned adjacent to a respective fluid port of the plurality of fluid ports, each fluid opening being configured to provide a path for communication of fluid from an adjacent fluid port to a corresponding fluid channel of the plurality of fluid channels.
5 . The microfluidic path device of claim 1 , the plurality of pressure ports being positioned in the first layer.
6 . The microfluidic path device of claim 1 , further comprising a plurality of pressure channels providing paths for communication of pneumatic pressure from the plurality of pressure ports to the pressure receiving sides of the plurality of chambers.
7 . The microfluidic path device of claim 6 , the third layer including a plurality of pressure openings, each pressure opening being positioned adjacent to a respective pressure port of the plurality of pressure ports, each pressure opening being configured to provide a path for communication of pneumatic pressure from an adjacent pressure port to a corresponding pressure channel of the plurality of pressure channels.
8 . The microfluidic path device of claim 1 , the elastic membrane being operable to vary a volume of each fluid-contacting side of the plurality of chambers.
9 . The microfluidic path device of claim 1 , the plurality of chambers including a first chamber and a second chamber, a first fluid channel of the plurality of fluid channels providing a path for communication of fluid from the fluid-contacting side of the first chamber to the fluid-contacting side of the second chamber, the elastic membrane being operable to form a closed valve in the first chamber to thereby prevent communication of fluid from the first chamber to the second chamber via the first fluid channel.
10 . The microfluidic path device of claim 1 , the plurality of chambers including a first chamber, a second chamber, and a third chamber, a first fluid channel of the plurality of fluid channels providing a path for communication of fluid from the fluid-contacting side of the first chamber to the fluid-contacting side of the second chamber, a second fluid channel of the plurality of fluid channels providing a path for communication of fluid from the fluid-contacting side of the second chamber to the fluid-contacting side of the third chamber, the elastic membrane being operable to peristaltically drive fluid from the second chamber to the third chamber via the second fluid channel.
11 . The microfluidic path device of claim 1 , the elastic membrane comprising a gas-permeable elastic portion positioned within the vacuum cap.
12 . The microfluidic path device of claim 11 , the gas-permeable elastic portion dividing the vacuum cap into a fluid-contacting side and a vacuum receiving side, the fluid-contacting side of the vacuum cap being in fluid communication with the fluid-contacting side of the one or more other chambers of the plurality of chambers.
13 . The microfluidic path device of claim 12 , the at least one projection being configured to prevent the gas-permeable elastic portion of the elastic membrane from sealing against the vacuum receiving side of the vacuum cap.
14 . The microfluidic path device of claim 1 , the first layer comprising a first plate, the second layer comprising a second plate.
15 . The microfluidic path device of claim 1 , one or both of the first layer or the second layer comprising a material that is at least substantially translucent to visible or ultraviolet light.
16 . The microfluidic path device of claim 1 , one or both of the first layer or the second layer comprising a transparent material.
17 . The microfluidic path device of claim 1 , one or both of the first layer or the second layer including a rigid material.
18 . The microfluidic path device of claim 1 , the at least one projection being generally centered relative to a width of the vacuum cap.
19 . A microfluidic path device comprising:
(a) a first layer;
(b) a second layer;
(c) a third layer interposed between the first layer and the second layer, the third layer including an elastic membrane, the elastic membrane having a gas-permeable portion;
(d) a plurality of chambers formed between the first layer and the second layer, the elastic membrane dividing each chamber into a fluid-contacting side in the first layer and a pressure-receiving side in the second layer;
(e) at least one fluid channel providing at least one path for communication of fluid among each fluid-contacting side of the plurality of chambers;
(f) a plurality of pressure ports providing paths for communication of pneumatic pressure to each pressure receiving side of the plurality of chambers; and
(g) a vacuum cap, the gas-permeable portion of the elastic membrane dividing the vacuum cap into a fluid-contacting side and a vacuum receiving side, the fluid-contacting side of the vacuum cap being in fluid communication with the fluid-contacting side of one or more chambers of the plurality of chambers, the vacuum cap being configured to provide removal of air from fluid communicated from the fluid-contacting side of the one or more chambers, the vacuum cap including at least one projection configured to prevent the gas-permeable portion of the elastic membrane from sealing against the vacuum receiving side of the vacuum cap.
20 . A microfluidic path device comprising:
(a) a first layer;
(b) a second layer;
(c) a third layer interposed between the first layer and the second layer, the third layer including an elastic membrane, the elastic membrane having a gas-permeable portion;
(d) a plurality of chambers formed between the first layer and the second layer, the elastic membrane dividing each chamber into a fluid-contacting side in the first layer and a pressure-receiving side in the second layer;
(e) at least one fluid channel providing at least one path for communication of fluid among each fluid-contacting side of the plurality of chambers;
(f) a plurality of pressure ports providing paths for communication of pneumatic pressure to each pressure receiving side of the plurality of chambers; and
(g) a vacuum cap, the gas-permeable portion of the elastic membrane dividing the vacuum cap into a fluid-contacting side and a vacuum receiving side, the fluid-contacting side of the vacuum cap being in fluid communication with the fluid-contacting side of one or more chambers of the plurality of chambers, the vacuum cap being configured to provide removal of air from fluid communicated from the fluid-contacting side of the one or more chambers, the vacuum cap including:
(i) an upper surface, and
(ii) a protrusion extending downwardly from the upper surface into the vacuum receiving side.