IP Library › Granted Patent US 12,246,326
Granted Patent B2
US 12,246,326 · App. 17/009,219 · Granted Mar 11, 2025

Systems and apparatus for holding plates

Inventors: David Maurice Cox (Pleasanton, CA); Stefanie Nishimura (Pleasanton, CA); Lauren Wong (Pleasanton, CA)
Assignee: 10x Genomics, Inc.
B01L9/523B01L2200/025
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Quick Facts
Patent No.
US 12,246,326
App. No.
17/009,219
Granted
Mar 11, 2025
Kind
B2
Abstract

Provided herein are devices and methods for securing microplates and/or microplates comprising tube strips. The devices provided herein may use a magnetic force to secure the microplates and/or the tube strips during heat sealing of wells or tubes.

Claims (30)

1. A microplate holder for securing a microplate comprising a proximal surface and a distal surface on opposite sides of the microplate, the microplate holder comprising:

a base plate comprising a proximal surface and a distal surface on opposite sides of the base plate, wherein the distal surface of the microplate rests adjacent the proximal surface of the base plate;

an aligner configured to accept the microplate, wherein the aligner comprises a proximal surface and a distal surface on opposite sides of the aligner and an attraction unit between the proximal surface and the distal surface of the aligner, wherein the attraction unit secures the distal surface of the aligner to the proximal surface of the base plate by magnetic force; and

a frame configured to rest directly on top of and contact the proximal surface of the aligner and the proximal surface of the microplate, wherein the frame comprises a plurality of projections that extend along a direction parallel to the proximal surface of the aligner and over the proximal surface of the microplate when present;

wherein the base plate, the aligner, the frame, and the microplate are separable from each other.

2. The microplate holder of claim 1 , wherein said base plate comprises an additional attraction unit that aligns with the attraction unit of the aligner.

3. The microplate holder of claim 2 , wherein said additional attraction unit is a magnet.

4. The microplate holder of claim 1 , wherein said base plate comprises one or more posts that are attractable towards said attraction unit.

5. The microplate holder of claim 4 , wherein said one or more posts comprise an additional attraction unit.

6. The microplate holder of claim 5 , wherein said additional attraction unit is a magnet.

7. The microplate holder of claim 4 , wherein said one or more posts are configured to abut at least one edge of said microplate.

8. The microplate holder of claim 4 , wherein said one or more posts restrict a lateral or longitudinal movement of said microplate.

9. The microplate holder of claim 1 , wherein said base plate comprises a first tab extending from a first longitudinal side of said base plate and a second tab extending from a second longitudinal side of said base plate.

10. The microplate holder of claim 1 , wherein the aligner comprises a lateral wall between the proximal and distal surfaces of the aligner, and a first arm extending from a first end of the lateral wall and a second arm extending from a second end of said lateral wall.

11. The microplate holder of claim 1 , wherein said frame comprises at least one frame hole configured to align with at least one aligner hole.

12. The microplate holder of claim 11 , wherein said at least one frame hole and said at least one aligner hole are configured to accept a screw or a securing pin.

13. The microplate holder of claim 12 , wherein said screw or said securing pin anchors said frame and said aligner together.

14. The microplate holder of claim 1 , wherein said frame comprises one or more snapping tabs extending from a side of said frame.

15. The microplate holder of claim 14 , wherein said one or more snapping tabs makes a mechanical contact with one or more aligner snapping tab apertures.

16. The microplate holder of claim 1 , wherein said frame comprises an additional attraction unit.

17. The microplate holder of claim 16 , wherein said additional attraction unit is a magnet.

18. The microplate holder of claim 16 , wherein said additional attraction unit is attractable towards said aligner to position said frame adjacent to said aligner and said proximal surface of said microplate.

19. The microplate holder of claim 1 , wherein said microplate holder prevents or restricts movement of said microplate.

20. The microplate holder of claim 19 , wherein said movement is a lateral movement, a longitudinal movement, or a proximal movement away from said base plate.

21. The microplate holder of claim 1 , wherein the plurality of projections aligns with rows of wells in the microplate.

22. A method of securing a microplate comprising a proximal surface and a distal surface on opposite sides of the microplate, the method comprising: attaching a microplate holder to the microplate, the microplate holder comprising:

a base plate comprising a proximal surface and a distal surface on opposite sides of the base plate, wherein the distal surface of the microplate rests adjacent the proximal surface of the base plate;

an aligner configured to accept the microplate, wherein the aligner comprises a proximal surface and a distal surface on opposite sides of the aligner and an attraction unit between the proximal surface and the distal surface of the aligner, wherein the attraction unit secures the distal surface of the aligner to the proximal surface of the base plate by magnetic force; and

a frame configured to rest directly on top of and contact the proximal surface of the aligner and the proximal surface of the microplate, wherein the frame comprises a plurality of projections that extend along a direction parallel to the proximal surface of the aligner and over the proximal surface of the microplate when present;

wherein the base plate, the aligner, the frame, and the microplate are separable from each other.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 2, 2020
From: COX, DAVID MAURICE; NISHIMURA, STEFANIE; WONG, LAUREN
To: 10X GENOMICS, INC.
Reel/Frame 053671/0265 →
Continuity (4)
Continuation PCTUS2019020425 · Mar 1, 2019
Provisional Application 62702702 · Jul 24, 2018
Provisional Application 62638036 · Mar 2, 2018
Related Publication 20200391215A1 · Dec 17, 2020
References Cited (92)
US 5658548A · Padhye et al. · 1997 [cited by applicant]
US 5705628A · Hawkins · 1998 [cited by applicant]
US 5897783A · Howe et al. · 1999 [cited by applicant]
US 6133436A · Koster et al. · 2000 [cited by applicant]
US 7718421B2 · Chen et al. · 2010 [cited by applicant]
US 9347056B2 · Saito et al. · 2016 [cited by applicant]
US 9975122B2 · Masquelier et al. · 2018 [cited by applicant]
US 10245587B2 · Masquelier et al. · 2019 [cited by applicant]
US 10544413B2 · Bharadwaj et al. · 2020 [cited by applicant]
US 10697000B2 · Belgrader et al. · 2020 [cited by applicant]
US 11135584B2 · Masquelier et al. · 2021 [cited by applicant]
US 20020174878A1 · Nisson et al. · 2002 [cited by applicant]
US 20030027203A1 · Fields · 2003 [cited by applicant]
US 20040040851A1 · Karger et al. · 2004 [cited by applicant]
US 20040165332A1 · Beson · 2004 [cited by examiner]
US 20040214175A9 · McKernan et al. · 2004 [cited by applicant]
US 20040228763A1 · Ingenhoven et al. · 2004 [cited by applicant]
US 20050013741A1 · a' Brassard · 2005 [cited by applicant]
US 20050025673A1 · Shimei · 2005 [cited by examiner]
US 20060094108A1 · Yoder et al. · 2006 [cited by applicant]
US 20070065808A1 · Bohm et al. · 2007 [cited by applicant]
US 20070117086A1 · Evans et al. · 2007 [cited by applicant]
US 20070251341A1 · Balmer · 2007 [cited by applicant]
US 20080003142A1 · Link et al. · 2008 [cited by applicant]
US 20080053205A1 · Pollack et al. · 2008 [cited by applicant]
US 20090305397A1 · Dodgson et al. · 2009 [cited by applicant]
US 20100006441A1 · Renaud et al. · 2010 [cited by applicant]
US 20110005978A1 · Bohm et al. · 2011 [cited by applicant]
US 20110114490A1 · Pamula et al. · 2011 [cited by applicant]
US 20110177592A1 · Faustman et al. · 2011 [cited by applicant]
US 20120015382A1 · Weitz et al. · 2012 [cited by applicant]
US 20120122714A1 · Samuels et al. · 2012 [cited by applicant]
US 20120190037A1 · Durin et al. · 2012 [cited by applicant]
US 20120196288A1 · Beer · 2012 [cited by applicant]
US 20130074944A1 · Van Gelder · 2013 [cited by applicant]
US 20130315800A1 · Yin · 2013 [cited by examiner]
US 20130323741A1 · Bernet et al. · 2013 [cited by applicant]
US 20140235506A1 · Hindson et al. · 2014 [cited by applicant]
US 20150031037A1 · Li et al. · 2015 [cited by applicant]
US 20150336096A1 · Smith et al. · 2015 [cited by applicant]
US 20150361418A1 · Reed · 2015 [cited by applicant]
US 20160298107A1 · O'Farrell et al. · 2016 [cited by applicant]
US 20170336306A1 · Miller et al. · 2017 [cited by applicant]
US 20180071741A1 · Kelly · 2018 [cited by examiner]
US 20180147574A1 · Dysli · 2018 [cited by examiner]
US 20180362963A1 · Stelling · 2018 [cited by applicant]
US 20190039034A1 · Siow et al. · 2019 [cited by applicant]
US 20190234977A1 · Antinjuntti · 2019 [cited by examiner]
US 20190329245A1 · Masquelier et al. · 2019 [cited by applicant]
US 20200115703A1 · Bharadwaj et al. · 2020 [cited by applicant]
US 20210032678A1 · Belgrader et al. · 2021 [cited by applicant]
US 20210187515A1 · Alimsijah et al. · 2021 [cited by applicant]
US 20210293693A1 · Bharadwaj et al. · 2021 [cited by applicant]
US 20220097045A1 · Masquelier et al. · 2022 [cited by applicant]
US 20220268795A1 · Alimsijah et al. · 2022 [cited by applicant]
EP 1348966A2 · 2003 [cited by applicant]
EP 1944368A1 · 2008 [cited by applicant]
EP 3262407A1 · 2018 [cited by applicant]
EP 3517974A1 · 2019 [cited by applicant]
EP 3605109A1 · 2020 [cited by applicant]
WO WO2006071770A2 · 2006 [cited by applicant]
WO WO2007140015A2 · 2007 [cited by applicant]
WO WO2010009365A1 · 2010 [cited by applicant]
WO WO2011059443A1 · 2011 [cited by applicant]
WO WO2012019765A1 · 2012 [cited by applicant]
WO WO2012156744A2 · 2012 [cited by applicant]
WO WO2014182835A1 · 2014 [cited by applicant]
WO WO2014210353A2 · 2014 [cited by applicant]
WO WO2015200717A2 · 2015 [cited by applicant]
WO WO2016137973A1 · 2016 [cited by applicant]
WO WO2016193758A1 · 2016 [cited by applicant]
WO WO2018213643A1 · 2018 [cited by applicant]
WO WO2019169347A1 · 2019 [cited by applicant]
WO WO2020123657A2 · 2020 [cited by applicant]
U.S. Appl. No. 17/471,603, filed Mar. 31, 2022, Masquelier et al. [cited by applicant]
International Search Report and Written Opinion for PCT Application No. PCT/US2019/020425, mailed May 13, 2019 (13 pages). [cited by applicant]
U.S. Appl. No. 17/242,802, Salmanzadeh. [cited by applicant]
U.S. Appl. No. 17/314,756, Salmanzadeh. [cited by applicant]
U.S. Appl. No. 17/332,371, Salmanzadeh et al. [cited by applicant]
U.S. Appl. No. 17/338,215, Salmanzadeh et al. [cited by applicant]
U.S. Appl. No. 17/587,861, Shah. [cited by applicant]
U.S. Appl. No. 17/851,416, Bharadwaj et al. [cited by applicant]
Anonymous: “Dynal MPC(TM)-S”, Oct. 13, 2008 (Oct. 13, 2008), XP055603532, Retrieved from the Internet on Jul. 9, 2019; URL:<https://www.veritastk.co.jp/products/pdf/120%2020D.Dynal_MPC-S%28rev005%29.pdf>. [cited by applicant]
Beneyton et al., “High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics,” Sci Rep. 6:27223 (Jun. 2016) (10 pages). [cited by applicant]
Brouzes et al., “Rapid and continuous magnetic separation in droplet microfluidic devices,” available in PMC Feb. 7, 2016, published in final edited form as: Lab Chip. 15(3):908-919 (2015) (23 pages). [cited by applicant]
Chokkalingam et al., “Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics,” Lab Chip. 13(24): 4740-4744 (2013). [cited by applicant]
Hu et al., “Efficient cell pairing in droplets using dual-color sorting,” Lab Chip. 15(20):3989-93 (2015). [cited by applicant]
Jo et al., “Magnetophoretic sorting of single cell-containing microdroplets,” Micromachines (Basel). 7(4): 56 (2016) (9 pages). [cited by applicant]
Klein et al., “Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells,” Cell. 161(5): 1187-1201 (2015) (May 21, 2015) (22 pages). [cited by applicant]
Lagus et al., “A review of the theory, methods and recent applications of high-throughput single-cell droplet microfluidics,” Journal of Physics D: Applied Physics. 46:114005 (2013) (21 pages). [cited by applicant]
Lennon et al., “A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454,” Genome Biol. 11(2):R15 (2010) (9 pages). [cited by applicant]
Shembekar et al., “Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics,” Lab Chip. 16(8):1314-31 (Mar. 2016). [cited by applicant]