IP Library › Granted Patent US 12,291,736
Granted Patent B2
US 12,291,736 · App. 18/600,576 · Granted May 6, 2025

Method for producing multi-hydroxy derivatives of polyunsaturated fatty acids

Inventors: Jeong Woo Seo (Daejeon, KR); Jong Jae Yi (Daejeon, KR); Sun Yeon Heo (Daejeon, KR); Young Bae Kim (Daejeon, KR); Chul Ho Kim (Daejeon, KR); Baek Rock Oh (Daejeon, KR); Jung Hyun Ju (Daejeon, KR); Hack Sun Choi (Jeju-si, KR)
Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
C12P7/6427A61P35/00C11C3/00C12N9/0069C12P7/6432C12P7/6434C12P17/02
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Quick Facts
Patent No.
US 12,291,736
App. No.
18/600,576
Granted
May 6, 2025
Kind
B2
Abstract

The present invention relates to a novel enzyme capable of producing multi-hydroxy derivatives from polyunsaturated fatty acids and a method for producing multi-hydroxy derivatives of polyunsaturated fatty acids using the same.

Claims (16)

1. An enzyme for producing di-hydroxy derivatives of polyunsaturated fatty acids, having the amino acid sequence of SEQ ID NO: 1.

2. The enzyme for producing the di-hydroxy derivatives of polyunsaturated fatty acids of claim 1 ,

wherein a substrate of the enzyme is docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA).

3. The enzyme for producing the di-hydroxy derivatives of polyunsaturated fatty acids of claim 2 ,

wherein the substrate of the enzyme is docosahexaenoic acid (DHA), and

wherein the di-hydroxy derivative of polyunsaturated fatty acids is a compound having the chemical structure of Chemical Formula 1:

4. A method for producing di-hydroxy derivatives of polyunsaturated fatty acids, comprising reacting the enzyme of claim 1 with polyunsaturated fatty acids as substrates.

5. The method for producing the di-hydroxy derivatives of polyunsaturated fatty acids of claim 4 , further comprising:

recovering the di-hydroxy derivative of polyunsaturated fatty acids from the reaction products of the enzyme and the polyunsaturated fatty acids.

6. The method for producing the di-hydroxy derivatives of polyunsaturated fatty acids of claim 4 ,

wherein the reaction is performed under conditions of 0° C. to 50° C. and pH 4 to pH 10.

7. The method for producing the di-hydroxy derivatives of polyunsaturated fatty acids of claim 4 ,

wherein the polyunsaturated fatty acid is docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA).

8. The method for producing the di-hydroxy derivatives of polyunsaturated fatty acids of claim 4 ,

wherein the polyunsaturated fatty acid is docosahexaenoic acid (DHA), and

wherein the di-hydroxy derivative of polyunsaturated fatty acids is a compound having the chemical structure of Chemical Formula 1:

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 11, 2024
From: SEO, JEONG WOO; YI, JONG JAE; HEO, SUN YEON; KIM, YOUNG BAE; KIM, CHUL HO; OH, BAEK ROCK; JU, JUNG HYUN; CHOI, HACK SUN
To: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
Reel/Frame 066710/0433 →
Priority Claims (4)
KR 10-2018-0043972 · Apr 16, 2018 · national
KR 10-2018-0051695 · May 4, 2018 · national
KR 10-2018-0142895 · Nov 19, 2018 · national
KR 10-2018-0142896 · Nov 19, 2018 · national
Continuity (3)
Division 17072696 · Oct 16, 2020
Continuation In Part PCTKR2019004583 · Apr 16, 2019
Related Publication 20240327883A1 · Oct 3, 2024
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