Pea albumin isolate from pea solubles and process for production thereof
The present invention provides a pea albumin isolate (PAI) extraction process comprising salt induced protein extraction from a pea soluble byproduct (pea solubles), the pea albumin isolate (PAI) produced by the PAI extraction process of the present disclosure and use thereof.
1 . A pea albumin isolate (PAI) extraction process comprising:
a) performing a salt-induced protein extraction from pea solubles by contacting the pea solubles with a solution:
i) at a pH of about 2 to about 5,
ii) at a temperature of about 18° C. to about 95° C.,
iii) for about 30 to about 60 minutes,
iii) with about 0.1 to about 5% concentration of sodium hexametaphosphate (SHMP), thereby precipitating the PAI; and
b) separating the PAI from the solution,
wherein the pea solubles are obtained from a pea protein isolate (PPI) extraction process comprising:
1) Protein extraction from aqueous solution comprising pea flour
i) at a pH of about 7 to 10,
ii) at a temperature of about 18° C. to 60° C.,
iii) for about 30 to 60 minutes for extraction,
2) separation of solids from the aqueous solution, producing a liquid fraction,
3) precipitation of a protein fraction from the liquid fraction comprising:
i) precipitating protein at a pH about 4 to 8, at a temperature of about 18° C. to 95° C. for about 5 to 60 minutes,
ii) optionally with SHMP;
4) separation of the liquid fraction from the protein fraction thereby forming the pea solubles.
2 . The process of claim 1 , wherein the protein extraction of step 1) comprises using Na, Ca, or Mg salt or a combination thereof.
3 . The process of claim 1 , wherein the salt concentration in the PPI extraction process is about 0.5% to about 5%.
4 . The process of claim 1 , wherein the salt induced protein extraction of step a) is performed at a temperature of about 50° C. to about 95° C.
5 . The process of claim 1 , wherein solids concentration of the pea solubles in step a) is about 3 to 20%.
6 . The process of claim 1 , wherein the pea solubles has a protein content in the range of about 80 to about 93 wt % (N×6.25) on a dry basis.
7 . The process of claim 1 , wherein the pea solubles contains about 5 to about 30% albumin.
8 . The process of claim 1 , wherein the pea solubles comprises PS-I albumin and PS-II albumin.
9 . The process of claim 1 , further comprising
c) washing the protein albumin isolate from step b).
10 . Process of claim 9 , further comprising
d) diluting the PAI in water to form a protein slurry.
11 . The process of claim 10 , further comprising
e) homogenizing and/or sterilizing the protein slurry.
12 . The process of claim 11 , further comprising
f) drying the protein slurry.
13 . The process of claim 1 , wherein the separating of step b) is performed by centrifugation.
14 . The process of claim 9 , wherein the washing of step c) is performed with water at the pH of the salt-induced protein extraction.