IP Library › Granted Patent US 12,729,402
Granted Patent B2
US 12,729,402 · App. 19/097,444 · Granted Sep 8, 2026

Methods and systems for processing polynucleotides

Inventors: Phillip Belgrader (Livermore, CA); Zachary Bent (Pleasanton, CA); Tarjei Sigurd Mikkelsen (Dublin, CA); Paul Ryvkin (San Jose, CA); Jessica Michele Terry (Pleasanton, CA); Elliott Meer (Pleasanton, CA); Daniel P. Riordan (San Francisco, CA)
Assignee: 10X GENOMICS, INC.
C12Q1/6874C12Q1/6806C12Q1/683C12Q2525/191C12Q2537/143C12Q2563/179
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,729,402
App. No.
19/097,444
Granted
Sep 8, 2026
Kind
B2
Abstract

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Claims (47)

1 . A method comprising:

(a) providing a partition comprising a plurality of barcode molecules coupled to a bead, and a plurality of analytes, where each of the plurality of barcode molecules comprises a barcode sequence that is the same for the plurality of barcode molecules; and

(b) hybridizing:

(i) a first barcode molecule of the plurality of barcode molecules to a first analyte that is mRNA via a first sequence that comprises a poly-T sequence that binds to a poly-A tail of the mRNA; and

(ii) a second barcode molecule of the plurality of barcode molecules to a second analyte that comprises a clustered regularly interspaced short palindromic repeat (CRISPR) ribonucleic acid (crRNA) or single guide ribonucleic acid (sgRNA) via a second sequence that is not a poly-T sequence.

2 . The method of claim 1 , further comprising:

(c) generating:

(i) a first barcoded nucleic acid molecule comprising: (1) the nucleic acid barcode sequence, or a complement thereof; and (2) a sequence of at least a portion of the first analyte, or a complement thereof; and

(ii) a second barcoded nucleic acid molecule comprising: (1) the nucleic acid barcode sequence, or a complement thereof; and (2) a sequence of at least a portion of the second analyte, or a complement thereof.

3 . The method of claim 2 , where (c) occurs outside of the partition.

4 . The method of claim 2 , further comprising:

(d) processing the first barcoded nucleic acid molecule and/or the second barcoded nucleic acid molecule, to identify the nucleic acid barcode sequence.

5 . The method of claim 4 , wherein (d) comprises subjecting the first barcoded nucleic acid molecule and/or the second barcoded nucleic acid molecule to sequencing to characterize the first analyte and/or the second analyte.

6 . The method of claim 1 , wherein the first barcode molecule and the second barcode molecule each comprise a random N-mer sequence.

7 . The method of claim 1 , wherein the bead is a gel bead.

8 . The method of claim 1 , wherein the bead comprises polyacrylamide.

9 . The method of claim 1 , wherein the partition is a partition among a plurality of partitions.

10 . The method of claim 9 , wherein the plurality of partitions comprises at least about 1,000, 5,000, 10,000, 50,000, 100,000, 500,000, 1,000,000, 5,000,000, 10,000,000, 50,000,000, 100,000,000, 500,000,000, or 1,000,000,000 partitions.

11 . The method of claim 10 , wherein the partition is a droplet, and the plurality of partitions is a plurality of droplets.

12 . The method of claim 10 , wherein the partition is a well, and the plurality of partitions is a plurality of wells.

13 . A method comprising:

(a) providing a plurality of droplets, where a droplet of the plurality of droplets comprises: a plurality of barcode molecules coupled to a gel bead, and a plurality of analytes, where each of the plurality of barcode molecules comprises:

(i) a barcode sequence that is the same for the plurality of barcode molecules, and

(ii) a random N-mer sequence; and

(b) hybridizing:

(i) a first barcode molecule of the plurality of barcode molecules to a first analyte that is mRNA via a first sequence that comprises a poly-T sequence that binds to a poly-A tail of the mRNA; and

(ii) a second barcode molecule of the plurality of barcode molecules to a second analyte that comprises clustered regularly interspaced short palindromic repeat (CRISPR) ribonucleic acid (crRNA) or single guide ribonucleic acid (sgRNA) via a second sequence that is not a poly-T sequence.

14 . The method of claim 13 , further comprising:

(c) generating:

(i) a first barcoded nucleic acid molecule comprising: (1) the nucleic acid barcode sequence, or a complement thereof; and (2) a sequence of at least a portion of the first analyte, or a complement thereof; and

(ii) a second barcoded nucleic acid molecule comprising: (1) the nucleic acid barcode sequence, or a complement thereof; and (2) a sequence of at least a portion of the second analyte, or a complement thereof.

15 . The method of claim 14 , wherein (c) occurs outside of the droplet.

16 . The method of claim 14 , further comprising:

(d) processing the first barcoded nucleic acid molecule and/or the second barcoded nucleic acid molecule, to identify the nucleic acid barcode sequence.

17 . The method of claim 16 , wherein (d) comprises subjecting the first barcoded nucleic acid molecule and/or the second barcoded nucleic acid molecule to sequencing to characterize the first analyte and/or the second analyte.

18 . The method of claim 1 , wherein the plurality of barcode molecules is releasably attached to the bead.

19 . The method of claim 13 , wherein the plurality of barcode molecules is releasably attached to the gel bead.

20 . A method comprising:

(a) providing a plurality of barcode molecules and a plurality of analytes, where each of the plurality of barcode molecules comprises a barcode sequence that is the same across barcode molecules of the plurality of barcode molecules;

(b) hybridizing:

(i) a first barcode molecule of the plurality of barcode molecules to a first analyte that is mRNA via a first sequence that comprises a poly-T sequence that binds to a poly-A tail of the mRNA; and

(ii) a second barcode molecule of the plurality of barcode molecules to a second analyte that comprises a clustered regularly interspaced short palindromic repeat (CRISPR) ribonucleic acid (crRNA) or single guide ribonucleic acid (sgRNA) via a second sequence that is not a poly-T sequence;

(c) generating:

(i) a first barcoded nucleic acid molecule comprising the nucleic acid barcode sequence, or a complement thereof; and a sequence of at least a portion of the first analyte, or a complement thereof;

(ii) a second barcoded nucleic acid molecule comprising the nucleic acid barcode sequence, or a complement thereof; and a sequence of at least a portion of the second analyte, or a complement thereof; and

(d) subjecting the first barcoded nucleic acid molecule and/or the second barcoded nucleic acid molecule to sequencing to characterize the first analyte and/or the second analyte.

21 . The method of claim 20 , wherein the first barcode molecule and the second barcode molecule each comprise a random N-mer sequence.

Assignments (3)
EMPLOYMENT AGREEMENT/ASSIGNMENT Recorded Jul 2, 2026
From: WU, INDIRA
To: 10X TECHNOLOGIES, INC.
Reel/Frame 075893/0848 →
CHANGE OF NAME Recorded Jul 2, 2026
From: 10X TECHNOLOGIES, INC.
To: 10X GENOMICS, INC.
Reel/Frame 075894/0242 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 22, 2026
From: BELGRADER, PHILLIP; BENT, ZACHARY; BHARADWAJ, RAJIV; GOPALAN, VIJAY KUMAR SREENIVASA; HARADA, JOSEPHINE; HINDSON, CHRISTOPHER; LENJI, MOHAMMAD RAHIMI; LUCERO, MICHAEL YBARRA; MCDERMOTT, GEOFFREY; MEER, ELLIOTT; MIKKELSEN, TARJEI SIGURD; O'KEEFFE, CHRISTOPHER JOACHIM; PFEIFFER, KATHERINE; PRICE, ANDREW D.; RYVKIN, PAUL; SAXONOV, SERGE; STUELPNAGEL, JOHN R.; TERRY, JESSICA MICHELE; WHEELER, TOBIAS DANIEL; ZIRALDO, SOLONGO BATJARGAL; BOUTET, STEPHAN CLAUDE; TAYLOR, SARAH; SRINIVAS, NIRANJAN
To: 10X GENOMICS, INC.
Reel/Frame 075168/0760 →
Continuity (16)
Continuation 18786209 · Jul 26, 2024
Continuation 17473760 · Sep 13, 2021
Continuation 17084481 · Oct 29, 2020
Continuation 16443638 · Jun 17, 2019
Continuation 16439675 · Jun 12, 2019
Continuation In Part 15933299 · Mar 22, 2018
Continuation 15720085 · Sep 29, 2017
Continuation In Part PCTUS2017068320 · Dec 22, 2017
Continuation In Part 15720085 · Sep 29, 2017
Continuation In Part PCTUS2018064600 · Dec 7, 2018
Continuation In Part 16107685 · Aug 21, 2018
Continuation In Part 16107685 · Aug 21, 2018
Provisional Application 62438341 · Dec 22, 2016
Provisional Application 62596557 · Dec 8, 2017
Provisional Application 62723960 · Aug 28, 2018
Related Publication 20250243539A1 · Jul 31, 2025
References Cited (400)
US 2797149A · Skeggs · 1957 [cited by applicant]
US 3047367A · Kessler · 1962 [cited by applicant]
US 3479141A · William et al. · 1969 [cited by applicant]
US 4124638A · Hansen · 1978 [cited by applicant]
US 4253846A · Smythe et al. · 1981 [cited by applicant]
US 4582802A · Zimmerman et al. · 1986 [cited by applicant]
US 5137829A · Nag et al. · 1992 [cited by applicant]
US 5149625A · Church et al. · 1992 [cited by applicant]
US 5185099A · Delpuech et al. · 1993 [cited by applicant]
US 5202231A · Drmanac et al. · 1993 [cited by applicant]
US 5270183A · Corbett et al. · 1993 [cited by applicant]
US 5413924A · Kosak et al. · 1995 [cited by applicant]
US 5418149A · Gelfand et al. · 1995 [cited by applicant]
US 5436130A · Mathies et al. · 1995 [cited by applicant]
US 5478893A · Ghosh et al. · 1995 [cited by applicant]
US 5489523A · Mathur · 1996 [cited by applicant]
US 5512131A · Kumar et al. · 1996 [cited by applicant]
US 5558071A · Ward et al. · 1996 [cited by applicant]
US 5585069A · Zanzucchi et al. · 1996 [cited by applicant]
US 5587128A · Wilding et al. · 1996 [cited by applicant]
US 5605793A · Stemmer et al. · 1997 [cited by applicant]
US 5605890A · Agrawal et al. · 1997 [cited by applicant]
US 5618711A · Gelfand et al. · 1997 [cited by applicant]
US 5695940A · Drmanac et al. · 1997 [cited by applicant]
US 5700642A · Monforte et al. · 1997 [cited by applicant]
US 5705628A · Hawkins · 1998 [cited by applicant]
US 5708153A · Dower et al. · 1998 [cited by applicant]
US 5736330A · Fulton · 1998 [cited by applicant]
US 5739036A · Parris · 1998 [cited by applicant]
US 5744311A · Fraiser et al. · 1998 [cited by applicant]
US 5756334A · Perler et al. · 1998 [cited by applicant]
US 5830663A · Embleton et al. · 1998 [cited by applicant]
US 5834197A · Parton · 1998 [cited by applicant]
US 5842787A · Kopf-Sill et al. · 1998 [cited by applicant]
US 5846719A · Brenner et al. · 1998 [cited by applicant]
US 5846727A · Soper et al. · 1998 [cited by applicant]
US 5851769A · Gray et al. · 1998 [cited by applicant]
US 5856174A · Lipshutz et al. · 1999 [cited by applicant]
US 5872010A · Karger et al. · 1999 [cited by applicant]
US 5897783A · Howe et al. · 1999 [cited by applicant]
US 5900481A · Lough et al. · 1999 [cited by applicant]
US 5942609A · Hunkapiller et al. · 1999 [cited by applicant]
US 5958703A · Dower et al. · 1999 [cited by applicant]
US 5965443A · Reznikoff et al. · 1999 [cited by applicant]
US 5994056A · Higuchi · 1999 [cited by applicant]
US 5997636A · Gamarnik et al. · 1999 [cited by applicant]
US 6033880A · Haff et al. · 2000 [cited by applicant]
US 6046003A · Mandecki · 2000 [cited by applicant]
US 6051377A · Mandecki · 2000 [cited by applicant]
US 6057107A · Fulton · 2000 [cited by applicant]
US 6057149A · Burns et al. · 2000 [cited by applicant]
US 6103537A · Ullman et al. · 2000 [cited by applicant]
US 6123798A · Gandhi et al. · 2000 [cited by applicant]
US 6133436A · Koester et al. · 2000 [cited by applicant]
US 6143496A · Brown et al. · 2000 [cited by applicant]
US 6159717A · Savakis et al. · 2000 [cited by applicant]
US 6171850B1 · Nagle et al. · 2001 [cited by applicant]
US 6172218B1 · Brenner · 2001 [cited by applicant]
US 6176962B1 · Soane et al. · 2001 [cited by applicant]
US 6207384B1 · Mekalanos et al. · 2001 [cited by applicant]
US 6258571B1 · Chumakov et al. · 2001 [cited by applicant]
US 6265552B1 · Schatz · 2001 [cited by applicant]
US 6291243B1 · Fogarty et al. · 2001 [cited by applicant]
US 6294385B1 · Goryshin et al. · 2001 [cited by applicant]
US 6296020B1 · McNeely et al. · 2001 [cited by applicant]
US 6297006B1 · Drmanac et al. · 2001 [cited by applicant]
US 6297017B1 · Schmidt et al. · 2001 [cited by applicant]
US 6303343B1 · Kopf-Sill · 2001 [cited by applicant]
US 6306590B1 · Mehta et al. · 2001 [cited by applicant]
US 6327410B1 · Walt et al. · 2001 [cited by applicant]
US 6355198B1 · Kim et al. · 2002 [cited by applicant]
US 6361950B1 · Mandecki · 2002 [cited by applicant]
US 6372813B1 · Johnson et al. · 2002 [cited by applicant]
US 6379929B1 · Burns et al. · 2002 [cited by applicant]
US 6406848B1 · Bridgham et al. · 2002 [cited by applicant]
US 6409832B2 · Weigl et al. · 2002 [cited by applicant]
US 6432290B1 · Harrison et al. · 2002 [cited by applicant]
US 6432360B1 · Church · 2002 [cited by applicant]
US 6485944B1 · Church et al. · 2002 [cited by applicant]
US 6492118B1 · Abrams et al. · 2002 [cited by applicant]
US 6511803B1 · Church et al. · 2003 [cited by applicant]
US 6524456B1 · Ramsey et al. · 2003 [cited by applicant]
US 6569631B1 · Pantoliano et al. · 2003 [cited by applicant]
US 6579851B2 · Goeke et al. · 2003 [cited by applicant]
US 6586176B1 · Trnovsky et al. · 2003 [cited by applicant]
US 6593113B1 · Tenkanen et al. · 2003 [cited by applicant]
US 6613752B2 · Kay et al. · 2003 [cited by applicant]
US 6632606B1 · Ullman et al. · 2003 [cited by applicant]
US 6632655B1 · Mehta et al. · 2003 [cited by applicant]
US 6670133B2 · Knapp et al. · 2003 [cited by applicant]
US 6723513B2 · Lexow · 2004 [cited by applicant]
US 6767731B2 · Hannah · 2004 [cited by applicant]
US 6800298B1 · Burdick et al. · 2004 [cited by applicant]
US 6806052B2 · Bridgham et al. · 2004 [cited by applicant]
US 6806058B2 · Jesperson et al. · 2004 [cited by applicant]
US 6859570B2 · Walt et al. · 2005 [cited by applicant]
US 6880576B2 · Karp et al. · 2005 [cited by applicant]
US 6884788B2 · Bulpitt et al. · 2005 [cited by applicant]
US 6890741B2 · Fan et al. · 2005 [cited by applicant]
US 6913935B1 · Thomas · 2005 [cited by applicant]
US 6915679B2 · Chien et al. · 2005 [cited by applicant]
US 6929859B2 · Chandler et al. · 2005 [cited by applicant]
US 6969488B2 · Bridgham et al. · 2005 [cited by applicant]
US 6974669B2 · Mirkin et al. · 2005 [cited by applicant]
US 7041481B2 · Anderson et al. · 2006 [cited by applicant]
US 7115400B1 · Adessi et al. · 2006 [cited by applicant]
US 7129091B2 · Ismagilov et al. · 2006 [cited by applicant]
US 7138267B1 · Jendrisak et al. · 2006 [cited by applicant]
US 7211654B2 · Gao et al. · 2007 [cited by applicant]
US 7268167B2 · Higuchi et al. · 2007 [cited by applicant]
US 7282370B2 · Bridgham et al. · 2007 [cited by applicant]
US 7294503B2 · Quake et al. · 2007 [cited by applicant]
US 7297485B2 · Bornarth et al. · 2007 [cited by applicant]
US 7316903B2 · Yanagihara et al. · 2008 [cited by applicant]
US 7323305B2 · Leamon et al. · 2008 [cited by applicant]
US 7329493B2 · Chou et al. · 2008 [cited by applicant]
US 7425431B2 · Church et al. · 2008 [cited by applicant]
US 7536928B2 · Kazuno · 2009 [cited by applicant]
US 7544473B2 · Brenner · 2009 [cited by applicant]
US 7604938B2 · Takahashi et al. · 2009 [cited by applicant]
US 7608434B2 · Reznikoff et al. · 2009 [cited by applicant]
US 7608451B2 · Cooper et al. · 2009 [cited by applicant]
US 7622076B2 · Davies et al. · 2009 [cited by applicant]
US 7622280B2 · Holliger et al. · 2009 [cited by applicant]
US 7638276B2 · Griffiths et al. · 2009 [cited by applicant]
US 7645596B2 · Williams et al. · 2010 [cited by applicant]
US 7666664B2 · Sarofim et al. · 2010 [cited by applicant]
US 7700325B2 · Cantor et al. · 2010 [cited by applicant]
US 7708949B2 · Stone et al. · 2010 [cited by applicant]
US 7709197B2 · Drmanac · 2010 [cited by applicant]
US 7745178B2 · Dong · 2010 [cited by applicant]
US 7745218B2 · Kim et al. · 2010 [cited by applicant]
US 7772287B2 · Higuchi et al. · 2010 [cited by applicant]
US 7776927B2 · Chu et al. · 2010 [cited by applicant]
US RE41780E · Anderson et al. · 2010 [cited by applicant]
US 7799553B2 · Mathies et al. · 2010 [cited by applicant]
US 7842457B2 · Berka et al. · 2010 [cited by applicant]
US 7901891B2 · Drmanac · 2011 [cited by applicant]
US 7910354B2 · Drmanac et al. · 2011 [cited by applicant]
US 7927797B2 · Nobile et al. · 2011 [cited by applicant]
US 7943671B2 · Herminghaus et al. · 2011 [cited by applicant]
US 7947477B2 · Schroeder et al. · 2011 [cited by applicant]
US 7960104B2 · Drmanac et al. · 2011 [cited by applicant]
US 7968287B2 · Griffiths et al. · 2011 [cited by applicant]
US 7972778B2 · Brown et al. · 2011 [cited by applicant]
US 8003312B2 · Krutzik et al. · 2011 [cited by applicant]
US 8008018B2 · Quake et al. · 2011 [cited by applicant]
US 8053192B2 · Bignell et al. · 2011 [cited by applicant]
US 8067159B2 · Brown et al. · 2011 [cited by applicant]
US 8101346B2 · Takahama · 2012 [cited by applicant]
US 8124404B2 · Alphey et al. · 2012 [cited by applicant]
US 8133719B2 · Drmanac et al. · 2012 [cited by applicant]
US 8137563B2 · Ma et al. · 2012 [cited by applicant]
US 8168385B2 · Brenner · 2012 [cited by applicant]
US 8252539B2 · Quake et al. · 2012 [cited by applicant]
US 8268564B2 · Roth et al. · 2012 [cited by applicant]
US 8273573B2 · Ismagilov et al. · 2012 [cited by applicant]
US 8278071B2 · Brown et al. · 2012 [cited by applicant]
US 8298767B2 · Brenner et al. · 2012 [cited by applicant]
US 8304193B2 · Ismagilov et al. · 2012 [cited by applicant]
US 8318433B2 · Brenner · 2012 [cited by applicant]
US 8318460B2 · Cantor et al. · 2012 [cited by applicant]
US 8329407B2 · Ismagilov et al. · 2012 [cited by applicant]
US 8337778B2 · Stone et al. · 2012 [cited by applicant]
US 8361299B2 · Sabin et al. · 2013 [cited by applicant]
US 8420386B2 · Ivics et al. · 2013 [cited by applicant]
US 8461129B2 · Bolduc et al. · 2013 [cited by applicant]
US 8563274B2 · Brenner et al. · 2013 [cited by applicant]
US 8592150B2 · Drmanac et al. · 2013 [cited by applicant]
US 8598328B2 · Koga et al. · 2013 [cited by applicant]
US 8603749B2 · Gillevet · 2013 [cited by applicant]
US 8658430B2 · Miller et al. · 2014 [cited by applicant]
US 8679756B1 · Brenner et al. · 2014 [cited by applicant]
US 8748094B2 · Weitz et al. · 2014 [cited by applicant]
US 8748102B2 · Berka et al. · 2014 [cited by applicant]
US 8765380B2 · Berka et al. · 2014 [cited by applicant]
US 8822148B2 · Ismagliov et al. · 2014 [cited by applicant]
US 8829171B2 · Steemers et al. · 2014 [cited by applicant]
US 8835358B2 · Fodor et al. · 2014 [cited by applicant]
US 8871444B2 · Griffiths et al. · 2014 [cited by applicant]
US 8889083B2 · Ismagilov et al. · 2014 [cited by applicant]
US 8927218B2 · Forsyth · 2015 [cited by applicant]
US 8975302B2 · Light et al. · 2015 [cited by applicant]
US 9005935B2 · Belyaev · 2015 [cited by applicant]
US 9012370B2 · Hong · 2015 [cited by applicant]
US 9012390B2 · Holtze et al. · 2015 [cited by applicant]
US 9017948B2 · Agresti et al. · 2015 [cited by applicant]
US 9029083B2 · Griffiths et al. · 2015 [cited by applicant]
US 9029085B2 · Agresti et al. · 2015 [cited by applicant]
US 9068210B2 · Agresti et al. · 2015 [cited by applicant]
US 9074251B2 · Steemers et al. · 2015 [cited by applicant]
US 9080211B2 · Grunenwald et al. · 2015 [cited by applicant]
US 9085798B2 · Chee · 2015 [cited by applicant]
US 9089844B2 · Hiddessen et al. · 2015 [cited by applicant]
US 9102980B2 · Brenner et al. · 2015 [cited by applicant]
US 9126160B2 · Ness et al. · 2015 [cited by applicant]
US 9133009B2 · Baroud et al. · 2015 [cited by applicant]
US 9150916B2 · Christen et al. · 2015 [cited by applicant]
US 9156010B2 · Colston et al. · 2015 [cited by applicant]
US 9175295B2 · Kaminaka et al. · 2015 [cited by applicant]
US 9194861B2 · Hindson et al. · 2015 [cited by applicant]
US 9216392B2 · Hindson et al. · 2015 [cited by applicant]
US 9238206B2 · Rotem et al. · 2016 [cited by applicant]
US 9238671B2 · Goryshin et al. · 2016 [cited by applicant]
US 9249460B2 · Pushkarev et al. · 2016 [cited by applicant]
US 9266104B2 · Link · 2016 [cited by applicant]
US 9273349B2 · Nguyen et al. · 2016 [cited by applicant]
US 9290808B2 · Fodor et al. · 2016 [cited by applicant]
US 9328382B2 · Drmanac et al. · 2016 [cited by applicant]
US 9347059B2 · Saxonov · 2016 [cited by applicant]
US 9371598B2 · Chee · 2016 [cited by applicant]
US 9388465B2 · Hindson et al. · 2016 [cited by applicant]
US 9410201B2 · Hindson et al. · 2016 [cited by applicant]
US 9417190B2 · Hindson et al. · 2016 [cited by applicant]
US 9486757B2 · Romanowsky et al. · 2016 [cited by applicant]
US 9498761B2 · Holtze et al. · 2016 [cited by applicant]
US 9500664B2 · Ness et al. · 2016 [cited by applicant]
US 9567631B2 · Hindson et al. · 2017 [cited by applicant]
US 9574226B2 · Gormley et al. · 2017 [cited by applicant]
US 9582877B2 · Fu et al. · 2017 [cited by applicant]
US 9593365B2 · Frisen et al. · 2017 [cited by applicant]
US 9623384B2 · Hindson et al. · 2017 [cited by applicant]
US 9637799B2 · Fan et al. · 2017 [cited by applicant]
US 9644204B2 · Hindson et al. · 2017 [cited by applicant]
US 9650407B2 · Gartner et al. · 2017 [cited by applicant]
US 9689024B2 · Hindson et al. · 2017 [cited by applicant]
US 9694361B2 · Bharadwaj et al. · 2017 [cited by applicant]
US 9695468B2 · Hindson et al. · 2017 [cited by applicant]
US 9701998B2 · Hindson et al. · 2017 [cited by applicant]
US 9727810B2 · Fodor et al. · 2017 [cited by applicant]
US 9764322B2 · Hiddessen et al. · 2017 [cited by applicant]
US 9824068B2 · Wong · 2017 [cited by applicant]
US 9856530B2 · Hindson et al. · 2018 [cited by applicant]
US 9868979B2 · Chee et al. · 2018 [cited by applicant]
US 9879313B2 · Chee et al. · 2018 [cited by applicant]
US 9946577B1 · Stafford et al. · 2018 [cited by applicant]
US 9951386B2 · Hindson et al. · 2018 [cited by applicant]
US 9957558B2 · Leamon et al. · 2018 [cited by applicant]
US 9975122B2 · Masquelier et al. · 2018 [cited by applicant]
US 10011872B1 · Belgrader et al. · 2018 [cited by applicant]
US 10017759B2 · Kaper et al. · 2018 [cited by applicant]
US 10030261B2 · Frisen et al. · 2018 [cited by applicant]
US 10030267B2 · Hindson et al. · 2018 [cited by applicant]
US 10041116B2 · Hindson et al. · 2018 [cited by applicant]
US 10053723B2 · Hindson et al. · 2018 [cited by applicant]
US 10059989B2 · Giresi et al. · 2018 [cited by applicant]
US 10071377B2 · Bharadwaj et al. · 2018 [cited by applicant]
US 10137449B2 · Bharadwaj et al. · 2018 [cited by applicant]
US 10144950B2 · Nolan · 2018 [cited by applicant]
US 10150117B2 · Bharadwaj et al. · 2018 [cited by applicant]
US 10150963B2 · Hindson et al. · 2018 [cited by applicant]
US 10150964B2 · Hindson et al. · 2018 [cited by applicant]
US 10150995B1 · Giresi et al. · 2018 [cited by applicant]
US 10151003B2 · Fan et al. · 2018 [cited by applicant]
US 10168328B2 · Berka · 2019 [cited by applicant]
US 10208343B2 · Hindson et al. · 2019 [cited by applicant]
US 10221436B2 · Hardenbol et al. · 2019 [cited by applicant]
US 10221442B2 · Hindson et al. · 2019 [cited by applicant]
US 10253364B2 · Hindson et al. · 2019 [cited by applicant]
US 10273541B2 · Hindson et al. · 2019 [cited by applicant]
US 10323278B2 · Belgrader et al. · 2019 [cited by applicant]
US 10323279B2 · Hindson et al. · 2019 [cited by applicant]
US 10338066B2 · Fan et al. · 2019 [cited by applicant]
US 10347365B2 · Wong et al. · 2019 [cited by applicant]
US 10357771B2 · Bharadwaj et al. · 2019 [cited by applicant]
US 10395758B2 · Schnall-Levin · 2019 [cited by applicant]
US 10400280B2 · Hindson et al. · 2019 [cited by applicant]
US 10428326B2 · Belhocine et al. · 2019 [cited by applicant]
US 10450607B2 · Hindson et al. · 2019 [cited by applicant]
US 10480029B2 · Bent et al. · 2019 [cited by applicant]
US 10533221B2 · Hindson et al. · 2020 [cited by applicant]
US 10544413B2 · Bharadwaj et al. · 2020 [cited by applicant]
US 10549279B2 · Bharadwaj et al. · 2020 [cited by applicant]
US 10550429B2 · Harada et al. · 2020 [cited by applicant]
US 10557158B2 · Hardenbol et al. · 2020 [cited by applicant]
US 10590244B2 · Delaney et al. · 2020 [cited by applicant]
US 10633691B2 · Wu · 2020 [cited by applicant]
US 10697008B2 · Blauwkamp et al. · 2020 [cited by applicant]
US 10745742B2 · Bent et al. · 2020 [cited by applicant]
US 10752949B2 · Hindson et al. · 2020 [cited by applicant]
US 10774374B2 · Frisen et al. · 2020 [cited by applicant]
US 10815525B2 · Lucero et al. · 2020 [cited by applicant]
US 10829815B2 · Bharadwaj et al. · 2020 [cited by applicant]
US 10837047B2 · Delaney et al. · 2020 [cited by applicant]
US 10858702B2 · Lucero et al. · 2020 [cited by applicant]
US 10874997B2 · Weitz et al. · 2020 [cited by applicant]
US 10995333B2 · Pfeiffer · 2021 [cited by applicant]
US 11332736B2 · Regev et al. · 2022 [cited by applicant]
US 11371094B2 · Ryvkin et al. · 2022 [cited by applicant]
US 11459607B1 · Terry et al. · 2022 [cited by applicant]
US 11467153B2 · Belhocine et al. · 2022 [cited by applicant]
US 11655499B1 · Pfeiffer · 2023 [cited by applicant]
US 11845983B1 · Belhocine et al. · 2023 [cited by applicant]
US 11851683B1 · Maheshwari et al. · 2023 [cited by applicant]
US 11851700B1 · Bava et al. · 2023 [cited by applicant]
US 11920183B2 · Bharadwaj et al. · 2024 [cited by applicant]
US 11952626B2 · Pfeiffer et al. · 2024 [cited by applicant]
US 12065688B2 · Bell · 2024 [cited by applicant]
US 12084715B1 · Lund · 2024 [cited by applicant]
US 12163179B2 · Bell et al. · 2024 [cited by applicant]
US 12169198B2 · Price et al. · 2024 [cited by applicant]
US 12188014B1 · Price et al. · 2025 [cited by applicant]
US 12235262B1 · Giresi · 2025 [cited by applicant]
US 12398262B1 · Gibbons · 2025 [cited by applicant]
US 20010020588A1 · Adourian et al. · 2001 [cited by applicant]
US 20010036669A1 · Jedrzejewski et al. · 2001 [cited by applicant]
US 20010041357A1 · Fouillet et al. · 2001 [cited by applicant]
US 20010044109A1 · Mandecki · 2001 [cited by applicant]
US 20010048900A1 · Bardell et al. · 2001 [cited by applicant]
US 20010053519A1 · Fodor et al. · 2001 [cited by applicant]
US 20020001856A1 · Chow et al. · 2002 [cited by applicant]
US 20020005354A1 · Spence et al. · 2002 [cited by applicant]
US 20020034737A1 · Drmanac · 2002 [cited by applicant]
US 20020043463A1 · Shenderov · 2002 [cited by applicant]
US 20020051971A1 · Stuelpnagel et al. · 2002 [cited by applicant]
US 20020051992A1 · Bridgham et al. · 2002 [cited by applicant]
US 20020058332A1 · Quake et al. · 2002 [cited by applicant]
US 20020065609A1 · Ashby · 2002 [cited by applicant]
US 20020068278A1 · Giese et al. · 2002 [cited by applicant]
US 20020089100A1 · Kawasaki · 2002 [cited by applicant]
US 20020092767A1 · Bjornson et al. · 2002 [cited by applicant]
US 20020113009A1 · O'Connor et al. · 2002 [cited by applicant]
US 20020119455A1 · Chan · 2002 [cited by applicant]
US 20020119536A1 · Stern · 2002 [cited by applicant]
US 20020127736A1 · Chou et al. · 2002 [cited by applicant]
US 20020131147A1 · Paolini et al. · 2002 [cited by applicant]
US 20020160518A1 · Hayenga et al. · 2002 [cited by applicant]
US 20020164820A1 · Brown · 2002 [cited by applicant]
US 20020166582A1 · O'Connor et al. · 2002 [cited by applicant]
US 20020172965A1 · Kamb et al. · 2002 [cited by applicant]
US 20020175079A1 · Christel et al. · 2002 [cited by applicant]
US 20020179849A1 · Maher et al. · 2002 [cited by applicant]
US 20030005967A1 · Karp · 2003 [cited by applicant]
US 20030007898A1 · Bohm et al. · 2003 [cited by applicant]
US 20030008285A1 · Fischer · 2003 [cited by applicant]
US 20030008323A1 · Ravkin et al. · 2003 [cited by applicant]
US 20030022231A1 · Wangh et al. · 2003 [cited by applicant]
US 20030027214A1 · Kamb · 2003 [cited by applicant]
US 20030027221A1 · Scott et al. · 2003 [cited by applicant]
US 20030028981A1 · Chandler et al. · 2003 [cited by applicant]
US 20030032141A1 · Nguyen et al. · 2003 [cited by applicant]
US 20030036206A1 · Chien et al. · 2003 [cited by applicant]
US 20030039978A1 · Hannah · 2003 [cited by applicant]
US 20030044777A1 · Beattie · 2003 [cited by applicant]
US 20030044836A1 · Levine et al. · 2003 [cited by applicant]
US 20030075446A1 · Culbertson et al. · 2003 [cited by applicant]
US 20030082587A1 · Seul et al. · 2003 [cited by applicant]
US 20030089605A1 · Timperman · 2003 [cited by applicant]
US 20030104466A1 · Knapp et al. · 2003 [cited by applicant]
US 20030108897A1 · Drmanac · 2003 [cited by applicant]
US 20030124509A1 · Kenis et al. · 2003 [cited by applicant]
US 20030149307A1 · Hai et al. · 2003 [cited by applicant]
US 20030170698A1 · Gascoyne et al. · 2003 [cited by applicant]
US 20030182068A1 · Battersby et al. · 2003 [cited by applicant]
US 20030207260A1 · Trnovsky et al. · 2003 [cited by applicant]
US 20030215862A1 · Parce et al. · 2003 [cited by applicant]
US 20040063138A1 · Mcginnis et al. · 2004 [cited by applicant]
US 20040068019A1 · Higuchi et al. · 2004 [cited by applicant]
US 20040081962A1 · Chen et al. · 2004 [cited by applicant]
US 20040101680A1 · Barber, Jr. · 2004 [cited by applicant]
US 20040101880A1 · Rozwadowski et al. · 2004 [cited by applicant]
US 20040132122A1 · Banerjee et al. · 2004 [cited by applicant]
US 20040224331A1 · Cantor et al. · 2004 [cited by applicant]
US 20040258701A1 · Dominowski et al. · 2004 [cited by applicant]
US 20050019839A1 · Jespersen et al. · 2005 [cited by applicant]
US 20050037397A1 · Mirkin et al. · 2005 [cited by applicant]
US 20050042625A1 · Schmidt et al. · 2005 [cited by applicant]
US 20050079510A1 · Berka et al. · 2005 [cited by applicant]
US 20050130188A1 · Walt et al. · 2005 [cited by applicant]
US 20050172476A1 · Stone et al. · 2005 [cited by applicant]
US 20050181379A1 · Su et al. · 2005 [cited by applicant]
US 20050202429A1 · Trau et al. · 2005 [cited by applicant]
US 20050202489A1 · Cho et al. · 2005 [cited by applicant]
US 20050221339A1 · Griffiths et al. · 2005 [cited by applicant]
US 20050244850A1 · Huang et al. · 2005 [cited by applicant]
US 20050250147A1 · Macevicz · 2005 [cited by applicant]
US 20050266582A1 · Modlin et al. · 2005 [cited by applicant]
US 20050272159A1 · Ismagilov et al. · 2005 [cited by applicant]
US 20050287572A1 · Mathies et al. · 2005 [cited by applicant]
US 20060002890A1 · Hersel et al. · 2006 [cited by applicant]
US 20060008799A1 · Cai et al. · 2006 [cited by applicant]
US 20060020371A1 · Ham et al. · 2006 [cited by applicant]
US 20060040382A1 · Heffron et al. · 2006 [cited by applicant]
US 20060073487A1 · Oliver et al. · 2006 [cited by applicant]
US 20060078888A1 · Griffiths et al. · 2006 [cited by applicant]
US 20060153924A1 · Griffiths et al. · 2006 [cited by applicant]
US 20060163385A1 · Link et al. · 2006 [cited by applicant]
US 20060177832A1 · Brenner · 2006 [cited by applicant]
US 20060177833A1 · Brenner · 2006 [cited by applicant]
US 20060199193A1 · Koo et al. · 2006 [cited by applicant]
US 20060240506A1 · Kushmaro et al. · 2006 [cited by applicant]
US 20060257893A1 · Takahashi et al. · 2006 [cited by applicant]
US 20060263888A1 · Fritz et al. · 2006 [cited by applicant]
US 20060275782A1 · Gunderson et al. · 2006 [cited by applicant]
US 20060286570A1 · Rowlen et al. · 2006 [cited by applicant]
US 20060292583A1 · Schneider et al. · 2006 [cited by applicant]
US 20070003442A1 · Link et al. · 2007 [cited by applicant]
US 20070020617A1 · Trnovsky et al. · 2007 [cited by applicant]
US 20070020640A1 · McCloskey et al. · 2007 [cited by applicant]
US 20070026401A1 · Hofmann et al. · 2007 [cited by applicant]