Granted Patent
B2
US 12,553,060 · App. 17/808,076 · Granted Feb 17, 2026
Genes, constructs and maize event DP-202216-6
Inventors:
Heather Marie Christensen (Ankeny, IA); Nathan David Coles (Johnston, IA); Olga Danilevskaya (Middleton, WI); Jeffrey Habben (Urbandale, IA); Mary A Rupe (Altoona, IA); Jeffrey R Schussler (Marion, IA); Bo Shen (Johnston, IA); Benjamin P Weers (Polk City, IA); Jingrui Wu (Johnston, IA)
Assignee:
PIONEER HI-BRED INTERNATIONAL, INC.
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Abstract
The compositions and methods disclosed relate to DNA compositions, plant cells, seeds, plant parts that relate to maize plants with increased grain yield trait. Also provided are assays for detecting the presence of the maize DP-202216-6 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
Claims (21)
1 . A commodity product produced from a transgenic maize plant comprising event DP-202216-6, a representative sample of a seed comprising the event has been deposited with American Type Culture Collection (ATCC) under Accession No. PTA-124653, and comprising a recombinant DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOS: 7, 8, 9, 10, 11, 12, 13, 14, 31, and 32, and full complements thereof, wherein detection of said recombinant DNA molecule in a sample derived from said commodity product is determinative that said commodity product was produced from said transgenic maize plant comprising event DP-202216-6.
2 . The commodity product of claim 1 , wherein said commodity product is selected from the group consisting of whole or processed seeds, animal feed, oil, meal, flour, flakes, bran, biomass, and fuel products.
3 . A method of producing the commodity product of claim 1 , the method comprising: (a) obtaining a maize plant or part thereof comprising transgenic maize event DP-202216-6, a representative sample of a seed comprising the event has been deposited with American Type Culture Collection (ATCC) under Accession No. PTA-124653; and (b) producing a maize commodity product from the maize plant or part thereof.
4 . A method of increasing grain yield of a population of maize plants in a field, the method comprising growing a population of maize plants comprising event DP-202216-6, a representative sample of a seed comprising the event has been deposited with American Type Culture Collection (ATCC) under Accession No. PTA-124653, in a field and thereby increasing grain yield of the population of maize plants compared to a control plant not comprising the event DP-202216-6.
5 . The method of claim 4 wherein the population of maize plants are grown under abiotic stress.
6 . The method of claim 5 , wherein the abiotic stress is low nitrogen.
7 . The method of claim 4 , wherein when grown under low nitrogen conditions, the population of maize plants comprising the event DP-202216-6, a representative sample of a seed comprising the event has been deposited with American Type Culture Collection (ATCC) under Accession No. PTA-124653, exhibits yield stability compared to the control population of plants grown under low nitrogen.
8 . The method of claim 4 , wherein the low nitrogen is about 25% to about 75% reduction in the amount of nitrogen normally applied to grow hybrid maize plants in the field.
9 . A recombinant DNA construct comprising an expression cassette, wherein the expression cassette in operable linkage comprises:
a) a maize gos2 promoter;
b) a maize ubiquitin gene 1 (ubiZM1) intron;
c) a maize MADS box gene encoding a maize protein of SEQ ID NO: 1;
d) a pinII terminator;
e) a maize ubiquitin gene 1 (ubiZM1) promoter;
f) a maize ubiquitin gene 1 (ubiZM1) 5′ UTR;
g) a maize ubiquitin gene 1 (ubiZM1) intron;
h) a mo-pat gene; and
i) a pinII terminator.
10 . A plant comprising the DNA construct of claim 9 .
11 . The plant of claim 10 , wherein said plant is a maize plant.
12 . Seed obtained from the plant of claim 9 .
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 21, 2022
From: CHRISTENSEN, HEATHER MARIE; COLES, NATHAN DAVID; DANILEVSKAYA, OLGA; HABBEN, JEFFREY; RUPE, MARY; SCHUSSLER, JEFFREY R; SHEN, BO; WEERS, BENJAMIN P; WU, JINGRUI
To: PIONEER HI-BRED INTERNATIONAL, INC.
Reel/Frame 060267/0712 →
Continuity (4)
Continuation
17047589
Provisional Application
62741529
· Oct 4, 2018
Provisional Application
62659579
· Apr 18, 2018
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