Granted Patent
B2
US 12,569,445 · App. 18/946,268 · Granted Mar 10, 2026
Particles comprising a therapeutic or diagnostic agent and suspensions and methods of use thereof
Inventors:
Chase Spenser Coffman (Newton, MA); Lyndon Fitzgerald Charles, Jr. (Medford, MA); Paul Brown (Boston, MA); Daniel Benjamin Dadon (East Boston, MA); Lisa Liu (Somerville, MA); Cory Robinson (Cambridge, MA)
Assignee:
Halozyme Hypercon, Inc.
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Abstract
The invention provides particles, compositions including the particles, and methods of making the particles using electrospray. In certain embodiments, the particles allow for high concentrations of a therapeutic or diagnostic agent to be delivered at low viscosity. Particles may also exhibit beneficial properties with respect to stability.
Claims (17)
1 . A composition comprising particles, wherein particles within the composition comprise (a) a therapeutic, wherein the therapeutic is a protein and (b) an excipient, wherein at least a portion of the excipient is localized at the particle surface, wherein the particles are in suspension in a non-aqueous liquid, wherein the non-aqueous liquid is an organic solvent or an ionic liquid and, wherein the therapeutic in the particles has 0.5 to 1.0 activity per unit, wherein the activity is the binding affinity of the therapeutic to a target.
2 . The composition of claim 1 , wherein the suspension has a viscosity from 0.27 to 200 cP.
3 . The composition of claim 1 , wherein the suspension comprises from 5 to 90% particles by volume.
4 . The composition of claim 1 , wherein the particles comprise a therapeutic at a concentration from 0.0001 to 1000 mg/ml.
5 . The composition of claim 1 , wherein the non-aqueous liquid is the organic solvent, which is selected from the group consisting of benzyl alcohol, benzyl benzoate, castor oil, coconut oil, corn oil, cottonseed oil, fish oil, grape seed oil, hazelnut oil, hydrogenated palm seed oil, olive oil, peanut oil, peppermint oil, safflower oil, sesame oil, soybean oil, sunflower oil, vegetable oil, walnut oil, polyethylene glycol, glycofurol, acetone, diglyme, dimethylacetamide, dimethyl isosorbide, dimethyl sulfoxide, ethanol, ethyl acetate, ethyl ether, ethyl lactate, isopropyl acetate, methyl acetate, methyl isobutyl ketone, methyl tertbutyl ether, N-methyl pyrrolidone, perfluorodecalin, 2-pyrrolidone, trigylcerides, tetrahydrofurfuryl alcohol, triglycerides of the fractionated plant fatty acids C8 and C10, propylene glycol diesters of saturated plant fatty acids C8 and C10, ethyl oleate, ethyl caprate, dibutyl adipate, fatty acid esters, hexanoic acid, octanoic acid, triacetin, diethyl glycol monoether, gamma-butyrolactone, eugenol, clove bud oil, citral, limonene, and any combination thereof.
6 . The composition of claim 1 , wherein the non-aqueous liquid is the organic solvent, which is selected from the group consisting of ethyl oleate and fatty acid esters.
7 . The composition of claim 1 , wherein the non-aqueous liquid is the ionic liquid, which comprises pyridinium, pyridazinium, pyrimidinium, pyrazinium, imidazolium, pyrazolium, thiazolium, oxazolium, triazolium, ammonium, sulfonium, halides, sulfates, sulfonates, carbonates, phosphates, bicarbonates, nitrates, acetates, PF6-, BF4-, triflate, nonaflate, bis(trifyl)amide, trifluoroacetate, heptafluorobutanoate, haloaluminate, or any combination thereof.
8 . The composition of claim 1 , wherein the particles have diameters from 0.1 to 1000 μm.
9 . The composition of claim 1 , wherein the particles have a polydispersity index from 0.05 to 0.9.
10 . The composition of claim 1 , wherein the excipient is a carbohydrate, a pH adjusting agent, a salt, a chelator, a mineral, a polymer, a surfactant, a protein stabilizer, an emulsifier, an antiseptic, an amino acid, an antioxidant, a protein, an organic solvent, a nutrient media, or any combination thereof.
11 . The composition of claim 10 , wherein the salt is present and is sodium chloride, calcium chloride, potassium chloride, sodium hydroxide, stannous chloride, magnesium sulfate, sodium glucoheptonate, sodium pertechnetate, or guanidine hydrochloride.
12 . The composition of claim 10 , wherein the protein is an antibody.
13 . The composition of claim 12 , wherein the antibody is human IgG or a monoclonal antibody.
14 . The composition of claim 10 , wherein the polymer is a poly(vinyl alcohol), poly(acrylic acid), poly(acrylamide), poly(ethylene oxide), or co-polymers or combinations of any two or more of them.
15 . The composition of claim 14 , wherein the polymer forms a shell around the particle.
16 . The composition of claim 14 , wherein the polymer does not form a uniform or continuous shell around the particle.
17 . A method of administering a therapeutic to a mammal, the method comprising administering an effective amount of a suspension or a dry formulation comprising particles to the mammal, wherein the suspension comprises a non-aqueous liquid, wherein the non-aqueous liquid is an organic solvent or an ionic liquid, and wherein particles within the suspension comprise (a) a therapeutic, wherein the therapeutic is a protein and (b) an excipient, wherein at least a portion of the excipient is localized at the particle surface and, wherein the therapeutic in the particles has 0.5 to 1.0 activity per unit, wherein the activity is the binding affinity of the therapeutic to a target.
Assignments (2)
CHANGE OF NAME
Recorded Feb 2, 2026
From: ELEKTROFI, INC.
To: HALOZYME HYPERCON, INC.
Reel/Frame 073662/0717 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 23, 2025
From: COFFMAN, CHASE; CHARLES, LYNDON; BROWN, PAUL; DADON, DANIEL BENJAMIN; LIU, LISA; ROBINSON, CORY
To: ELEKTROFI, INC.
Reel/Frame 072193/0558 →
Continuity (8)
Continuation
16463238
Provisional Application
62476173
· Mar 24, 2017
Provisional Application
62476190
· Mar 24, 2017
Provisional Application
62468727
· Mar 8, 2017
Provisional Application
62468735
· Mar 8, 2017
Provisional Application
62425399
· Nov 22, 2016
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