Granted Patent B2

US 12,241,109 · App. 18/886,925 · Granted Mar 4, 2025

Inventors: Viviane Lanquar (San Carlos, CA); Magi El-Richani (San Francisco, CA); Leyla Hathwaik (South San Francisco, CA); Yaxin Wang (South San Francisco, CA)

Assignee: Nobell Foods, Inc.

C12P21/06A23C20/00A23J3/10C07K14/4732C12N9/6424C12N9/6483C12Y304/21009C07K2319/00

Abstract

Claims (39)

1. A method of making a food composition, said method comprising the steps of:

a) providing a plant cell expressing:

i) a heterologous casein protein; and

ii) a heterologous Fam20C kinase protein;

b) obtaining the heterologous casein protein from the plant cell; and

c) processing the heterologous casein protein into a food composition, thereby making the food composition.

2. The method of claim 1 , wherein the plant cell is from a dicot.

3. The method of claim 2 , wherein the dicot is selected from the group consisting of arabidopsis, tobacco, tomato, potato, sweet potato, cassava, alfalfa, lima bean, pea, chickpea, soybean, carrot, strawberry, lettuce, oak, maple, walnut, rose, mint, squash, daisy, quinoa, buckwheat, mung bean, cow pea, lentil, lupin, peanut, fava bean, French bean, mustard, and cactus.

4. The method of claim 2 , wherein the dicot is soybean.

5. The method of claim 1 , wherein the plant cell is from a monocot.

6. The method of claim 5 , wherein the monocot is selected from the group consisting of maize, rice, oat, wheat, barley, and sorghum.

7. The method of claim 1 , wherein the heterologous Fam20C kinase protein comprises a sequence with at least 90% sequence identity to SEQ ID NO: 821.

8. The method of claim 1 , wherein the heterologous Fam20C kinase protein comprises SEQ ID NO: 821.

9. The method of claim 1 , wherein the heterologous Fam20C kinase protein comprises a sequence with at least 90% sequence identity to amino acids 94-586 of SEQ ID NO: 821.

10. The method of claim 1 , wherein the heterologous Fam20C kinase protein comprises amino acids 94-586 of SEQ ID NO: 821.

11. The method of claim 1 , wherein the heterologous casein protein is selected from the group consisting of: α-S1 casein, α-S2 casein, β-casein, κ-casein, and para-κ-casein.

12. The method of claim 1 , wherein the heterologous casein protein is a mature truncated casein, lacking a signal peptide.

13. The method of claim 1 , wherein the heterologous casein protein is within a fusion protein comprising a second milk protein or a zein protein.

14. The method of claim 13 , wherein the second milk protein is selected from the group consisting of an α-S1 casein, an α-S2 casein, a β-casein, a κ-casein, a para-κ-casein, a β-lactoglobulin, an α-lactalbumin, a lysozyme, a lactoferrin, a lactoperoxidase, a serum albumin, and an immunoglobulin.

15. The method of claim 1 , wherein the heterologous Fam20C kinase protein comprises a plant signal peptide.

16. A method of producing a plant-based casein mimic, said method comprising the steps of:

a) providing a plant cell expressing:

i) a heterologous casein protein; and

ii) a heterologous Fam20C kinase protein; and

b) separating the heterologous casein protein from the plant cell.

17. The method of claim 16 , wherein the plant cell is from a dicot.

18. The method of claim 17 , wherein the dicot is selected from the group consisting of arabidopsis, tobacco, tomato, potato, sweet potato, cassava, alfalfa, lima bean, pea, chickpea, soybean, carrot, strawberry, lettuce, oak, maple, walnut, rose, mint, squash, daisy, quinoa, buckwheat, mung bean, cow pea, lentil, lupin, peanut, fava bean, French bean, mustard, and cactus.

19. The method of claim 17 , wherein the dicot is soybean.

20. The method of claim 16 , wherein the plant cell is from a monocot.

21. The method of claim 20 , wherein the monocot is selected from the group consisting of maize, rice, oat, wheat, barley, and sorghum.

22. The method of claim 16 , wherein the heterologous Fam20C kinase protein comprises a sequence with at least 90% sequence identity to SEQ ID NO: 821.

23. The method of claim 16 , wherein the heterologous Fam20C kinase protein comprises SEQ ID NO: 821.

24. The method of claim 16 , wherein the heterologous Fam20C kinase protein comprises a sequence with at least 90% sequence identity to amino acids 94-586 of SEQ ID NO: 821.

25. The method of claim 16 , wherein the heterologous Fam20C kinase protein comprises amino acids 94-586 of SEQ ID NO: 821.

26. The method of claim 16 , wherein the heterologous casein protein is selected from the group consisting of: α-S1 casein, α-S2 casein, β-casein, κ-casein, and para-κ-casein.

27. The method of claim 16 , wherein the heterologous casein protein is a mature truncated casein, lacking a signal peptide.

28. The method of claim 16 , wherein the heterologous casein protein is within a fusion protein comprising a second milk protein or a zein protein.

29. The method of claim 28 , wherein the second milk protein is selected from the group consisting of an α-S1 casein, an α-S2 casein, a β-casein, a κ-casein, a para-κ-casein, a β-lactoglobulin, an α-lactalbumin, a lysozyme, a lactoferrin, a lactoperoxidase, a serum albumin, and an immunoglobulin.

30. The method of claim 16 , wherein the heterologous Fam20C kinase protein comprises a plant signal peptide.

Assignments (1)

Continuity (14)

References Cited (400)